Tissue Culture

Originally published in 1983, Plants from Test Tubes was one of the first how-to books on plant tissue culture or "cloning". It continues to be an internationally popular primer. New to this third edition is in-depth information on culture contaminants, a tutorial on the use of the microscope, and a discussion of the role of tissue culture as an indispensable tool in modern biotechnology. Plants from Test Tubes remains the most accessible and practical book on the subject. The book is divided into two sections. The first, "The Basics of Tissue Culture", begins with a discussion of the historical and botanical background of micropropagation. A short course in chemistry lays the foundation for media preparation instructions. Sterile technique and culture care are described in detail. Advice is offered on how to deal with possible problems with cultures, including such topics as vitrification, lack of growth, and contamination. Section II, "Culture Guide to Selected Plants", provides recipes for propagating 54 varieties of ferns, conifers, and flowering plants - an increase of 15 percent from the previous edition. An extensive bibliography and a large appendix of suppliers of chemicals and equipment complete this comprehensive edition.

In this third edition the authors have written a guide to the essentials of plant tissue culture. The book takes the reader through a graded series of experimental protocols and provides an introductory review of each topic. There are discussions of aseptic techniques, and nutritional components of media. Subsequent chapters cover such topics as callus formation, organogenesis, xylem cell differentiation, root cultures, cell suspensions, micropropagation by buds, somatic embryogenesis, protoplast culture and fusion, secondary metabolite production, and quantitation of procedures. A glossary, commercial sources of supplies, and the formulations of culture media are also included. This new edition has been completely revised and updated. The organization has been improved, and new illustrations have been added, together with new experiments on such topics as potato callus formation, and embryo culture. This book will be useful to graduate students and professionals in plant science, forestry, agronomy, and horticulture.

Plant tissue culture - Plant tissue culture, also called micropropagation, is a practice used to propagate plants under sterile conditions, often to produce clones of a plant. Different techniques in plant tissue culture may offer certain advantages over traditional methods of propagation, including:

Tissue culture - A tissue culture is the growth of cells (tissue) separate from the organism. In 1907 the American zoologist Ross G Harrison demonstrated the growth of frog nerve cell processes in a medium of clotted lymph.

Organ culture - Organ culture is a development from tissue culture methods of research, the organ culture is able to accurately model functions of an organ in various states and conditions by the use of the actual in vitro organ itself.

William Summerlin - William Summerlin worked at the Sloan-Kettering Institute for Cancer Research in New York. He claimed that he could transplant tissue from unrelated animals by keeping the tissue in culture for four to six weeks.

tissueculture

Plant Micropropagation - Plant Micropropagation Plants from Test Tubes Originally published in 1983, Plants from Test Tubes was one of the first how-to books on plant tissue culture or cloning. It continues to be an internationally popular primer. New to this third edition is in-depth information on culture contaminants, a tutorial on the use of the microscope, plant micropropagation and a discussion of the role of tissue culture as an indispensable tool in modern biotechnology. Plants from Test Tubes remains the most ...

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